Journal: BMC Cancer

Article Title: Comparative study of clinical features, pathology, and immunophenotype between HIV-related cutaneous and visceral Kaposi’s sarcoma

doi: 10.1186/s12885-026-15910-w

Figure Lengend Snippet: Immunohistochemical Features. The left panel shows the cutaneous group, specifically from the shoulder skin, and the right panel shows the visceral group, specifically from an inguinal lymph node. Both panels display the immunohistochemical expression of PD-1, PD-L1, CyclinD1, RB, and BCL-2. Among these, PD-1 showed positivity in lymphocytes within the tumor stroma; PD-L1 showed membrane positivity in tumor cells; CyclinD1 and RB showed nuclear positivity; and BCL-2 showed cytoplasmic positivity

Article Snippet: Sections were incubated with rabbit anti-human antibodies against CyclinD1 (SP4), Rb (13A10), BCL-2 (124), P53 (BP-53-12), PD-1 ((2E5), PD-L1 (ZR3), Ki-67 (MIB-1), and, CD34 (QBEnd10) (from Gene Tech, Shanghai, China), HHV8 (13B10) (from zsbio, Beijing, China) for 25 min at room temperature.

Techniques: Immunohistochemical staining, Expressing, Membrane

Journal: Frontiers in Physiology

Article Title: Lifelong Football Training: Effects on Autophagy and Healthy Longevity Promotion

doi: 10.3389/fphys.2019.00132

Figure Lengend Snippet: Effects of lifelong football training on the expression of key markers involved in autophagy. Protein expression levels of HSP70, HSP90, and ATG5-ATG12 complex, involved in autophagy process, of anti-apoptotic Bcl-2, and of PSMD13 subunit of the 19S regulator complex were analyzed by Western Blotting in muscle biopsies from 15 controls (CG, black bars) to15 veterans subjects (VPG, gray bars). GAPDH served as loading control. Representative blots are reported for each protein analyzed. Data were expressed as percentage of CG expression. Comparison between groups was determined by ANOVA and represent the means (±SEM) of three different experiments. Differences were considered significant at ∗ p < 0.05 vs CG.

Article Snippet: The membranes were immunoblotted using rabbit polyclonal antibodies against autophagy related 5 homolog (ATG5), autophagy related 12 homolog (ATG12), mouse monoclonal antibodies against Heat Shock Protein 90 (HSP90), Heat Shock Protein 70 (HSP70), B-cell lymphoma 2 (Bcl-2) (Elabscience, 1:500), monoclonal rabbit antibody proteasome 26S subunit non-ATPase 13 (PSMD13) (Abcam, 1:1000), monoclonal mouse antibody against glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) (1:1000; Santa-Cruz Biotechnology Inc.).

Techniques: Expressing, Western Blot, Control, Comparison

Journal: OncoTargets and therapy

Article Title: FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma

doi: 10.2147/OTT.S190934

Figure Lengend Snippet: Primer sequences

Article Snippet: Then, membranes were washed by TBST 3 times and incubated overnight at 4°C with primary antibodies against FAM111B (PA5-58474; Invitrogen), BAG3 (AB47124; Abcam), BCL2 (TA806639S; Origene), β-actin (8H10D10; Cell Signaling Technology) at a 1:1,000 dilution in 5% BSA.

Techniques:

Journal: OncoTargets and therapy

Article Title: FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma

doi: 10.2147/OTT.S190934

Figure Lengend Snippet: FAM111B might affect the expression of p53 related protein BAG3, BCL2 and CCNB1. ( A ) The result of KEGG bioinformatics analysis showed that genes similar to FAM111B expression were enriched in the cell cycle and p53 signaling pathway. ( B ) The mRNA expression of G2M regulate proteins CCNB1 and CDC25C have a close correlation with that of FAM111B. ( C ) Bioinformatics analysis indicated that the expression of FAM111B correlated with BAG3. ( D ) The expression of G2M regulated protein CCNB1 and apoptosis-related proteins BAG3 and BCL-2 was significantly reduced when FAM111B was knocked down. ( E ) Knockdown of BAG3 had no significant effect on the expression of FAM111B. ( F ) FAM111B might participate in the regulation of tumor by p53 signaling pathway while promoting the malignancy of LUAD through cell cycle and apoptosis. Abbreviation: LUAD, lungadenocarcinoma.

Article Snippet: Then, membranes were washed by TBST 3 times and incubated overnight at 4°C with primary antibodies against FAM111B (PA5-58474; Invitrogen), BAG3 (AB47124; Abcam), BCL2 (TA806639S; Origene), β-actin (8H10D10; Cell Signaling Technology) at a 1:1,000 dilution in 5% BSA.

Techniques: Expressing, Knockdown

Journal: Cancer Communications

Article Title: Phosphatidylserine released from apoptotic cells in tumor induces M2‐like macrophage polarization through the PSR‐STAT3‐JMJD3 axis

doi: 10.1002/cac2.12272

Figure Lengend Snippet: Apoptotic tumor cells correlated with M2‐like macrophages in tumor. (A) Representative immunofluorescence images of apoptotic tumor cells (Cleaved Caspase‐3 + , green) and macrophages (CD163 + , red) in human colon cancer specimens are shown (left: more apoptotic tumor cells; right: fewer apoptotic cells). (B‐D) Correlation analyses for the number of apoptotic tumor cells and macrophages in human colon cancer (B), breast cancer (C), and lung cancer (D) are shown. (E) Representative immunofluorescence images of apoptotic cells (Cleaved Caspase‐3 + , green) and macrophages (F4/80 + , red) in mouse CT26 colon cancer specimens are shown (left: more apoptotic tumor cells; right: fewer apoptotic cells). (F‐H) Correlation analyses for the number of apoptotic tumor cells and macrophages in CT26 (F), 4T1 (G), or LL2 (H) subcutaneous tumor are shown the number of apoptotic tumor cells or macrophages per specimen were calculated by the mean number counted in ten HPFs. The Pearson correlation coefficieI(r) and significance levels ( P ) are presented. (I) 72 h after peritoneal injection of different CT26 cells (Control, Bcl2, or shBcl2), the accumulation of M2 macrophages (CD45 + CD11b + F4/80 + CD206 + ) in the peritoneal cavity were detected by flow cytometry. (J) Infiltration of M2 type macrophages (CD11b + F4/80 + CD206 + ) in CT26 malignant ascites with or without DNR treatment (1 mg/kg, once) are shown. (K) Chemotherapy‐induced apoptotic CT26 cells (DNR‐CT26) elevated CD206 + macrophage accumulation in peritoneal cavity. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 calculated using a two‐tailed unpaired Student's t‐test Abbreviations: HPF, high power field; DNR, daunorubicin.

Article Snippet: The murine Bcl2 gene (GenBank accession number NM_009741) was cloned by OriGene Technologies Inc (Rockville, MD, USA).

Techniques: Immunofluorescence, Injection, Control, Flow Cytometry, Two Tailed Test